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Download PDF by Herbert E. Spiegel: Advances in Clinical Chemistry, Vol. 37

By Herbert E. Spiegel

ISBN-10: 0120103370

ISBN-13: 9780120103379

This quantity, edited for the final time via H.E. Spiegel, keeps the target of increasing the highbrow horizon of medical chemistry. The fields of analytical, anatomical, sub-cellular and molecular sciences are all represented during this quantity. * particular experiences by means of practising scientists * Covers a huge diversity of scientific chemistry on a theoretical and sensible foundation * comprises effortless to learn chapters combining technological know-how and views in a altering medical panorama

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Extra info for Advances in Clinical Chemistry, Vol. 37

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Cytometric Bead Array (CBA) The CBA (BD Pharmingen) is a series of spectrally discrete, uniform-size microparticles that can be used simultaneously to detect different soluble analytes including cytokines in a single sample on the basis of different color using flow cytometry. This series of particles with discrete fluorescence intensities can simultaneously detect a panel of six human cytokines (IFN-γ , TNF-α, IL-2, IL-4, IL-5, and IL-10) (C9, C16) (Fig. 3; see color insert). This newly developed method can save more time, consume less sample, and achieve similar sensitivity (pg/ml) and reproducibility compared with ELISA.

Activated cells are then stained with peridinin chlorophyll protein (PerCP)-conjugated, CD4specific monoclonal antibody and treated with a lysing solution. , anti-IFN-γ and IL-4 antibodies). , % IFN-γ and % IL-4) can be analyzed using flow cytometry (Fig. 2). 2. Cytometric Bead Array (CBA) The CBA (BD Pharmingen) is a series of spectrally discrete, uniform-size microparticles that can be used simultaneously to detect different soluble analytes including cytokines in a single sample on the basis of different color using flow cytometry.

J. Biol. Chem. 271, 695– 701 (1996). H6. Harandi, A. , Interleukin-12 (IL-12) and IL-18 are important in innate defense against genital herpes simplex virus type 2 infection in mice but are not required for the development of acquired gamma interferon-mediated protective immunity. J. Virol. 75, 6705–6709 (2001). H7. Hart, L. , Krishnan, V. , Adcock, I. , Barnes, P. , and Chung, K. , Activation and localization of transcription factor, nuclear factor-κB, in asthma. Am. J. Respir. Crit. Care Med.

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Advances in Clinical Chemistry, Vol. 37 by Herbert E. Spiegel


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