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Get Applied Biochemistry and Bioengineering. Enzyme Technology PDF

By Lemuel Wingard (Eds.)

ISBN-10: 0120411024

ISBN-13: 9780120411023

ISBN-10: 0323156010

ISBN-13: 9780323156011

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Extra info for Applied Biochemistry and Bioengineering. Enzyme Technology

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2 + 2 2. Subtilisin Novo 2 (Subtilisin BPN) Subtilisin Novo or Subtilisin B P N is produced by Bacillus liquefaciens. This new species is separated from B. subtilis amylo­ (Welker 52 Knud Aunstrup and Campbell, 1967), because the strains used for industrial enzyme production have many properties in common, primarily the properties o f their extracellular enzymes which were different from those o f the neotype o f B . subtilis, the Marburg strain. B. amyloliquefaciens has been used for industrial enzyme produc­ tion for over 5 0 years.

T h e protease hydrolyzes all proteins o f the medium, and at the end o f the fermentation period the protease is practically the only protein dissolved in the broth. T h e yield is usually high. It is possible Production of Extracellular Enzymes 51 to obtain a yield o f enzyme protein o f over 1 0 % o f the initial protein content o f the medium. T h e enzyme is primarily sold in the form o f dust-free granulates for detergent use. Commercial granulates contain 1-5% active enzyme protein. T h e enzyme is also stable in liquid form, and the enzyme content o f liquid preparations is about 2 % .

Lilly Thurston, C. F. (1972). Process Biochem. August, 18. Toda, K. (1976). Biotechnol. Bioeng. 18, 1103. Wang, D. I. C , and Fewkes, R. C. J. (1976). Dev. Ind. Microbiol. 18, 39. Wang, D. I. C , Cooney, C. , Demain, A. , Humphrey, A. , and Lilly, M. D. (1979). " Wiley, New York. Wimpenny, J . W. T. (1969). Symp. Soc. Gen. Microbiol. 19, 161. , and Vas, K. (1968). Biotechnol. Bioeng. 10, 45. Production, Isolation, and Economics of Extracellular Enzymes Knud Aunstrup Novo Research Institute Bagsvaerd, Denmark I.

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Applied Biochemistry and Bioengineering. Enzyme Technology by Lemuel Wingard (Eds.)


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